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two step approach first staining with ifitm1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc two step approach first staining with ifitm1
    A. RT-qPCR analysis of IFN-1 RNA levels. B . Secreted IFN-1 protein levels detected in culture supernatant via HEK-Blue SEAP reporter assay. C . RT-qPCR analysis of IRDS genes. Dots indicate genes known to be transcribed by u-ISGF3. D . Intracellular flow cytometry of <t>IFITM1,</t> total STAT1, and pSTAT1(y701). One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*).
    Two Step Approach First Staining With Ifitm1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/two step approach first staining with ifitm1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    two step approach first staining with ifitm1 - by Bioz Stars, 2026-06
    86/100 stars

    Images

    1) Product Images from "Interferon Beta Drives Therapy Resistance in a Patient-Derived Model of High-Grade Serous Ovarian Cancer"

    Article Title: Interferon Beta Drives Therapy Resistance in a Patient-Derived Model of High-Grade Serous Ovarian Cancer

    Journal: bioRxiv

    doi: 10.64898/2026.01.27.699126

    A. RT-qPCR analysis of IFN-1 RNA levels. B . Secreted IFN-1 protein levels detected in culture supernatant via HEK-Blue SEAP reporter assay. C . RT-qPCR analysis of IRDS genes. Dots indicate genes known to be transcribed by u-ISGF3. D . Intracellular flow cytometry of IFITM1, total STAT1, and pSTAT1(y701). One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*).
    Figure Legend Snippet: A. RT-qPCR analysis of IFN-1 RNA levels. B . Secreted IFN-1 protein levels detected in culture supernatant via HEK-Blue SEAP reporter assay. C . RT-qPCR analysis of IRDS genes. Dots indicate genes known to be transcribed by u-ISGF3. D . Intracellular flow cytometry of IFITM1, total STAT1, and pSTAT1(y701). One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*).

    Techniques Used: Quantitative RT-PCR, Reporter Assay, Flow Cytometry

    A. The IC50 of SE and CR cells was significantly higher following IFNβ treatment. B . Morphology change was determined by measuring the aspect ratio (width/legth) of 60 cells from each sample (20 cells per photo, 2 photos per well, 3 wells). Scale bar = 50µm. C . BrdU staining revealed cell cycle arrest in G0-G1 following IFNβ treatment, as measured by flow cytometry. Data represent one representative replicate. D . Proliferation was detected by counting individual wells of parental and IFNβ treated cells over time. E . RT-qPCR was used to analyze STAT1, IFITM1, and PLSCR1 following 6 passages and 12 weeks following consistent low-level IFNβ treatment. F . Intracellular flow cytometry was used to measure STAT1 and pSTAT1 protein levels following chronic IFNβ treatment. One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*) and p ≤ 0.01 (**).
    Figure Legend Snippet: A. The IC50 of SE and CR cells was significantly higher following IFNβ treatment. B . Morphology change was determined by measuring the aspect ratio (width/legth) of 60 cells from each sample (20 cells per photo, 2 photos per well, 3 wells). Scale bar = 50µm. C . BrdU staining revealed cell cycle arrest in G0-G1 following IFNβ treatment, as measured by flow cytometry. Data represent one representative replicate. D . Proliferation was detected by counting individual wells of parental and IFNβ treated cells over time. E . RT-qPCR was used to analyze STAT1, IFITM1, and PLSCR1 following 6 passages and 12 weeks following consistent low-level IFNβ treatment. F . Intracellular flow cytometry was used to measure STAT1 and pSTAT1 protein levels following chronic IFNβ treatment. One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*) and p ≤ 0.01 (**).

    Techniques Used: BrdU Staining, Flow Cytometry, Quantitative RT-PCR



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    two step approach first staining with ifitm1  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc two step approach first staining with ifitm1
    A. RT-qPCR analysis of IFN-1 RNA levels. B . Secreted IFN-1 protein levels detected in culture supernatant via HEK-Blue SEAP reporter assay. C . RT-qPCR analysis of IRDS genes. Dots indicate genes known to be transcribed by u-ISGF3. D . Intracellular flow cytometry of <t>IFITM1,</t> total STAT1, and pSTAT1(y701). One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*).
    Two Step Approach First Staining With Ifitm1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/two step approach first staining with ifitm1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    two step approach first staining with ifitm1 - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    A. RT-qPCR analysis of IFN-1 RNA levels. B . Secreted IFN-1 protein levels detected in culture supernatant via HEK-Blue SEAP reporter assay. C . RT-qPCR analysis of IRDS genes. Dots indicate genes known to be transcribed by u-ISGF3. D . Intracellular flow cytometry of IFITM1, total STAT1, and pSTAT1(y701). One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*).

    Journal: bioRxiv

    Article Title: Interferon Beta Drives Therapy Resistance in a Patient-Derived Model of High-Grade Serous Ovarian Cancer

    doi: 10.64898/2026.01.27.699126

    Figure Lengend Snippet: A. RT-qPCR analysis of IFN-1 RNA levels. B . Secreted IFN-1 protein levels detected in culture supernatant via HEK-Blue SEAP reporter assay. C . RT-qPCR analysis of IRDS genes. Dots indicate genes known to be transcribed by u-ISGF3. D . Intracellular flow cytometry of IFITM1, total STAT1, and pSTAT1(y701). One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*).

    Article Snippet: Cells were also stained using a two-step approach first staining with IFITM1 (99969S; Cell Signaling Technology) diluted in 0.5% BSA for 1 hour at room temperature before rinsing and staining with AlexaFluorTM 647 goat anti-rabbit IgG (H+L) secondary antibody (A21245; Fisher Scientific) at room temperature for 30 minutes, washed, and resuspended in FACS stain.

    Techniques: Quantitative RT-PCR, Reporter Assay, Flow Cytometry

    A. The IC50 of SE and CR cells was significantly higher following IFNβ treatment. B . Morphology change was determined by measuring the aspect ratio (width/legth) of 60 cells from each sample (20 cells per photo, 2 photos per well, 3 wells). Scale bar = 50µm. C . BrdU staining revealed cell cycle arrest in G0-G1 following IFNβ treatment, as measured by flow cytometry. Data represent one representative replicate. D . Proliferation was detected by counting individual wells of parental and IFNβ treated cells over time. E . RT-qPCR was used to analyze STAT1, IFITM1, and PLSCR1 following 6 passages and 12 weeks following consistent low-level IFNβ treatment. F . Intracellular flow cytometry was used to measure STAT1 and pSTAT1 protein levels following chronic IFNβ treatment. One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*) and p ≤ 0.01 (**).

    Journal: bioRxiv

    Article Title: Interferon Beta Drives Therapy Resistance in a Patient-Derived Model of High-Grade Serous Ovarian Cancer

    doi: 10.64898/2026.01.27.699126

    Figure Lengend Snippet: A. The IC50 of SE and CR cells was significantly higher following IFNβ treatment. B . Morphology change was determined by measuring the aspect ratio (width/legth) of 60 cells from each sample (20 cells per photo, 2 photos per well, 3 wells). Scale bar = 50µm. C . BrdU staining revealed cell cycle arrest in G0-G1 following IFNβ treatment, as measured by flow cytometry. Data represent one representative replicate. D . Proliferation was detected by counting individual wells of parental and IFNβ treated cells over time. E . RT-qPCR was used to analyze STAT1, IFITM1, and PLSCR1 following 6 passages and 12 weeks following consistent low-level IFNβ treatment. F . Intracellular flow cytometry was used to measure STAT1 and pSTAT1 protein levels following chronic IFNβ treatment. One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*) and p ≤ 0.01 (**).

    Article Snippet: Cells were also stained using a two-step approach first staining with IFITM1 (99969S; Cell Signaling Technology) diluted in 0.5% BSA for 1 hour at room temperature before rinsing and staining with AlexaFluorTM 647 goat anti-rabbit IgG (H+L) secondary antibody (A21245; Fisher Scientific) at room temperature for 30 minutes, washed, and resuspended in FACS stain.

    Techniques: BrdU Staining, Flow Cytometry, Quantitative RT-PCR